ProteinGOLDisafastandsensitivefluorescentdyeforvisualizationandquantitationofproteinsseparatedby1-Dor2-DSDS-PAGE.Itcomesasa100xstocksolutionthatissimplydilutedwithwaterbytheusertoitsworkingconcentration.

ProteinGOLDisnormallylowfluorescentbutemitsstrongfluorescence(brightgoldencolor)asboundtoproteins.Thestainingprocedureisasimpletwo-stepprotocol(fixandstain)thatcanbecompletedinaslittleas30minutes.Gelstobestainedarefixedwithethanol/aceticacidsolutionpriortostainingwithProteinGOLDsolution.Adestainstepisnotnormallyrecommended,butmaybeemployedtoreducebackground,simplybyagitatingthegelinwaterfor1-5minutes.GelsstainedwithProteinGOLDfluorescentgelstainmaybedirectlyvisualizedwithavarietyofdifferentUV-basedfluorescenceimagingsystems.Themaximumemissionwavelengthofprotein-boundProteinGOLDisnear570nm.ProteinGOLDgivesexceptionalsensitivityandwidedynamicrangeforproteindetection.TheboundProteinGOLDdyeiseasilyremovedfromtheproteinbyimmersingthegelinsufficientwater,thusitiswellcompatIBLewithsubsequentenzymaticdigestionandmassspectrometryforproteomicsapplications.

Stainedgelsmaybestoredinstainsolutioninthedarkat2-8°C;imagingsensitivitymightbemoderatelyenhancedafter4°Cstorageofthestainedgel.