ChemiCLisahorserADIshperoxidasesubstrateoptimizedforchemiluminescentWesternblotsimagedusingX-rayfilm.ChemiCLrequiresaslittleastentimeslessantibodythanothersubstrates,allowingyoutosavepreciousantibodiesandsamples.Additionally,theChemiCLsignalislonglasting,allowingmultipleexposurestobecarriedoutwithoutsubstantialsignaldecay.
Kitincludes:ChemiCLLuminol/enhancersolutionandChemiCLPeroxideChemiluminescentperoxidesolution.
200ml,goodfor25miniblots.
Features:
- StrongersignalthanAmersham™ECLorPierce™ECL
- Detectlowpgproteinamounts
- Useuptoten-timeslessantibody
Duplicateslotblotscontainingserialdilutionsoftransferrinwereprobedwiththeantibodydilutionsshown,anddetectedwitheitherChemiCLorPierceECL(ThermoScientific)accordingtothemanufacturers’instructions.Theblotswereimagedsimultaneouslyonthesamepieceoffilm.ChemiCLproducedthesamesignalwithtentimeslessprimaryandfivetimeslesssecondaryantibody.