ChemiFPisthemostsensitiveHRPsubstrateavailablefromGelCompanyforchemiluminescentWesternblotting.Withattomolesensitivityandalong-lastingsignal,ChemiFPallowsyoutodetectbandsnotvisualizedwithothersubstrates.Highsignal-to-noiseandalargedynamicrangemakeitidealforquantifyinglow-intensitybands.ChemifluorescenceemissionallowsChemiFPtobeimagedbychemifluorescenceimagersinadditiontotrADItionalCCDimagersandfilm.
100ml,for1000cm2membrane
Features
•Detectlow-abundanceproteins
•Lowbackgroundforhighsignal-to-noiseratio
•Long-lastingsignalforflexIBLeimaging
•ImagechemiluminescencebyCCDorfilm,imagechemifluorescencewithlaser-basedfluorescenceimagingsystems.
SUPPORTMATERIALS
ChemiFPProtocol(67.5kB)
ResultsachievedbyusingamembranelipidstripfromEchelonBiosciencesandfollowingtheprotocolbelow:
1.Incubatethestripwith0.5ug/mlofrecombinantprotein1hRT
2.Primaryantibody1:10001hRT
3.Secondaryantibody1:50001hRT
4.Developingwith“ChemiFP"
5.ImagingwithOmegaLumCat"Chemo"and20secexposure
