TheArrayMixcanhold1-4microscopeslidescontainedwithin16-24wellsectioningchambers(chamberssoldseperately)foratotalofupto96arrays.Eachslidemakesdirectcontactwithathermalblocktomaintainaccuratetemperaturecontrolfrom15ºCupto90ºC,withatoleranceof0.1ºC.Eachofthethreeslidessitsonanorbitalshaker.TheslidesorbitaroundasmallrADIusatanadjustablespeed,fromalowof300revolutionsperminutetoamaximumof900revolutionsperminute.Theorbitalshakingallowsforuseoflowconcentrationordilutetargetsinhybridization,whichsavesmoneywhenusingexpensivepeptidesorantibodiesforarrayexperiments.
Mixingduringhybridizationcanalsobeanessentialstepforsuccessinarrayexperiments.Mixingduringhybridizationcanalsobeanessentialstepforsuccessinarrayexperiments,asreferencedbythefollowingpublications:
Kineticsofantigenbindingtoantibodymicrospots:stronglimitationbymasstransporttothesurface.KusnezowW,SyagailoYV,RufferS,KleninK,SebaldW,HoheiselJD,GauerC,GoychukI.Proteomics.2006Feb;6(3):794-803.
Optimaldesignofmicroarrayimmunoassaystocompensateforkineticlimitations:theoryandexperiment.KusnezowW,SyagailoYV,RufferS,BaudenstielN,GauerC,HoheiselJD,WildD,GoychukI.MolCellProteomics.2006Sep;5(9):1681-96.
Afterhybridizations,theArraySlidechamberscanberemovedandseparatedforscanningorfurtherprocessingofthemicroarrayslides.ArraySlidechamberssoldseparately.
