TheGreenDNAprovidesaneasy2-stepmethodtostainDNAbandsfromDNAelectrophoresis.ThisuniquereagentensuresDNAtobestainedwithahighsensitivityandgoodquality. GreenDNAisanext-generationDNA-bindingdyewithfeaturesidealforuseinquantitativereal-timePCR(qPCR)andmanyotherapplications.ThedyewasdesignedbytakingintoconsiderationseveralessentialdyepropertiesrelevanttoPCR,includingPCRinhibition,safety,andstABIlityandfluorescencespectraofthedye.Ethidiumbromide(EtBr),whichcandetect1-5ngofthedouble-strandedDNA(dsDNA)intheagarosegelanalysis,hasbeenthemostcommondyeusedfornucleicacidgelstaining.However,severaldrawbacksofEtBrhavebeenunderstood,includingthatEtBrisamutagen/carcinogenandpresentsahighriskofinducingcancer.Moreover,theultraviolet(UV)lightusedtoIlluminateEtBr-DNAcompoundswillprobablyresultinskinoreyedamagetotheuserifmisconducted.It’salsonotedthatexposuretotheUVlightmightcausechemicalmodificationsoftheDNAsamplesinthegel,suchastheformationofTTdimers,leADIngtochallengeswiththesubsequentDNAmanipulations.Reducedefficiencyoftransformationwasobservedbyourscientists,afterconductingligationwiththeDNAsamplesisolatedfromthegelexposedtoalongerperiodofUVillumination.

AscomparedwiththeEtBr,the GreenDNAshowsamuchhighersensitivityunderthe254nmtransilluminationandisoneofthemostsensitivestainsfordetectingdsDNAintheagarosegel.Inadditiontothehighsensitivity,the GreenDNAbringsamorereliableandsaferuserexperiencesincethestainedgelcanbevisualizedwiththeblue-lighttransilluminator,thusavoidingtheriskofskin/eyedamageaswellasreducingthesideeffectsofDNAmodificationcausedbytheUVlight.

• Easydisposal:Passedenvironmentalsafetytestsfordirectdisposaldownthedrainorinregulartrash.
• Ultra-sensitive:MoresensativethanSYBRGreenI
• FlexIBLefordifferentprocedures:Canbeusedforeitherprecastorpostgelstaining.
• Simpletouse:Verysimpleproceduresforprecastandpostgelstainings.
• PerfectCompatibilitywithaStandardUVoraBluelightTransilluminator:GreenDNAreplacesEtBrwithnoopticalsettingchange;GreenDNAreplacesSYBRGreenIwithnoopticalsettingchange.

1KBDNALadder(250-10kbp)was2Xserialdiluted(from2to128dilution,respectively)andloadedinthe1%agarosegel.Afterelectrophoresis,the gelwasstainedfor10minwithGreenDNA(Fig.1a)orwithSYBR®GreenIfromanotherBrand(Fig.1b).TheleftstainedgelswereobservedwiththeUV254 transilluminatorandphotographedbyCCDcamera,andtherightstainedgelswereobservedwiththeBlueLighttransilluminator.

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MSDS(66.55kB)
Protocol(44.82kB)